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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
22/02/2022 |
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Data da última atualização: |
24/02/2022 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
VASQUES, G. de M.; RODRIGUES, H. M.; TAVARES, S. R. de L.; HERNANI, L. C.; OLIVEIRA, R. P. de. |
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Afiliação: |
GUSTAVO DE MATTOS VASQUES, CNPS; HUGO MACHADO RODRIGUES, UFRRJ; SILVIO ROBERTO DE LUCENA TAVARES, CNPS; LUIS CARLOS HERNANI, CNPS; RONALDO PEREIRA DE OLIVEIRA, CNPS. |
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Título: |
An optimized sample for assessing soil property variations across the field and within management zones efficiently. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
In: PEDOMETRICS BRAZIL, 2., 2021, Rio de Janeiro. Annals [...]. Rio de Janeiro: Embrapa Solos, 2022. Não paginado. Evento online. |
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Idioma: |
Inglês |
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Conteúdo: |
An optimized sampling design to assess soil property variation across the field and within management zones is proposed and validated in a 72-ha crop field in southeastern Brazil. An optimized sample (18 sites) was derived by spatial simulated annealing from proximal sensor covariates. Soil properties were measured at 0-10 cm and validated against those measured at 72 sites on a regular grid. The optimized and regular grid samples had equal global spatial trend models and means for soil clay, pH and exchangeable Ca, Mg and K, and different ones for organic C and available P. Within zones, equal means between sampling designs were found for all soil properties in the ?North? zone, and for most properties in the other two zones. Soil property correlations against proximal sensor variables were honored by the optimized samples in most cases, both globally and within zones. The optimized soil sample reduces costs while keeping most soil information for guiding management decisions. |
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Palavras-Chave: |
Proximal soil sensing; Spatial simulated annealing; Spatial trends. |
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Thesagro: |
Agricultura de Precisão; Sensoriamento Remoto. |
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Thesaurus Nal: |
Precision agriculture; Remote sensing. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/231718/1/An-optimized-sample-for-assessing-soil-property-variations-2022.pdf
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Marc: |
LEADER 01870nam a2200241 a 4500
001 2140307
005 2022-02-24
008 2022 bl uuuu u00u1 u #d
100 1 $aVASQUES, G. de M.
245 $aAn optimized sample for assessing soil property variations across the field and within management zones efficiently.$h[electronic resource]
260 $aIn: PEDOMETRICS BRAZIL, 2., 2021, Rio de Janeiro. Annals [...]. Rio de Janeiro: Embrapa Solos, 2022. Não paginado. Evento online.$c2022
520 $aAn optimized sampling design to assess soil property variation across the field and within management zones is proposed and validated in a 72-ha crop field in southeastern Brazil. An optimized sample (18 sites) was derived by spatial simulated annealing from proximal sensor covariates. Soil properties were measured at 0-10 cm and validated against those measured at 72 sites on a regular grid. The optimized and regular grid samples had equal global spatial trend models and means for soil clay, pH and exchangeable Ca, Mg and K, and different ones for organic C and available P. Within zones, equal means between sampling designs were found for all soil properties in the ?North? zone, and for most properties in the other two zones. Soil property correlations against proximal sensor variables were honored by the optimized samples in most cases, both globally and within zones. The optimized soil sample reduces costs while keeping most soil information for guiding management decisions.
650 $aPrecision agriculture
650 $aRemote sensing
650 $aAgricultura de Precisão
650 $aSensoriamento Remoto
653 $aProximal soil sensing
653 $aSpatial simulated annealing
653 $aSpatial trends
700 1 $aRODRIGUES, H. M.
700 1 $aTAVARES, S. R. de L.
700 1 $aHERNANI, L. C.
700 1 $aOLIVEIRA, R. P. de
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Registro original: |
Embrapa Solos (CNPS) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
29/05/2023 |
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Data da última atualização: |
25/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PEREIRA, B. M.; ARRAES, F.; MARTINS, A. C. Q.; ALVES, N. S. F.; MELO, B. P.; MORGANTE, C. V.; SARAIVA, M. A. P.; SA, M. F. G. de; GUIMARAES, P. M.; BRASILEIRO, A. C. M. |
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Afiliação: |
BRUNA MEDEIROS PEREIRA, UNIVERSIDADE DE BRASÍLIA; FABRÍCIO ARRAES, UNIVERSIDADE DE BRASILIA; ANDRESSA CUNHA QUINTANA MARTINS, UNIVERSIDADE CATOLICA DE BRASILIA; NAYARA SABRINA FREITAS ALVES, UNIVERSIDADE FEDERAL DO PARANÁ; BRUNO PAES MELO, LONGPING HIGH TECH; CAROLINA VIANNA MORGANTE, CPATSA; MARIO ALFREDO PASSOS SARAIVA, INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA; MARIA FATIMA GROSSI DE SA, CENARGEN; PATRICIA MESSEMBERG GUIMARAES, CENARGEN; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN. |
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Título: |
A novel soybean hairy root system for gene functional validation. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plos One, v. 18, n. 5, e0285504, 2023. |
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DOI: |
https://doi. org/10.1371/journal.pone.0285504 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes. |
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Conteúdo: |
Agrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean. MenosAgrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suita... Mostrar Tudo |
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Palavras-Chave: |
Agrobacterium rhizogenes; Transgenic soybean. |
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Thesagro: |
Glycine Max; Melhoramento Genético Vegetal; Meloidogyne spp; Soja. |
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Thesaurus NAL: |
Soybeans. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154066/1/A-novel-soybean-hairy-root-system-for-gene-functional-validation-2023.pdf
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Marc: |
LEADER 02598naa a2200337 a 4500
001 2154066
005 2024-04-25
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi. org/10.1371/journal.pone.0285504$2DOI
100 1 $aPEREIRA, B. M.
245 $aA novel soybean hairy root system for gene functional validation.$h[electronic resource]
260 $c2023
500 $aNa publicação: Maria Fátima Grossi-de-Sa; Patricia Messenberg Guimaraes.
520 $aAgrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis -expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean.
650 $aSoybeans
650 $aGlycine Max
650 $aMelhoramento Genético Vegetal
650 $aMeloidogyne spp
650 $aSoja
653 $aAgrobacterium rhizogenes
653 $aTransgenic soybean
700 1 $aARRAES, F.
700 1 $aMARTINS, A. C. Q.
700 1 $aALVES, N. S. F.
700 1 $aMELO, B. P.
700 1 $aMORGANTE, C. V.
700 1 $aSARAIVA, M. A. P.
700 1 $aSA, M. F. G. de
700 1 $aGUIMARAES, P. M.
700 1 $aBRASILEIRO, A. C. M.
773 $tPlos One$gv. 18, n. 5, e0285504, 2023.
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Embrapa Semiárido (CPATSA) |
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